10k high density protein microarray platform Search Results


prrsv m protein polyclonal antibody  (Bioss)
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10k high density protein microarray platform  (OriGene)
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OriGene 10k high density protein microarray platform
10k High Density Protein Microarray Platform, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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super marathon microarrayer  (Arrayjet Limited)
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scanner luxscan 10k  (CapitalBio Corporation)
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luxscan (10k microarray scanner)  (CapitalBio Corporation)
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CapitalBio Corporation luxscan (10k microarray scanner)
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tomato 10k microarray genechip  (Thermo Fisher)
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anti-gst/ anti-his antibody-dylight tm 550  (Rockland Immunochemicals)
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dylight 550 conjugated anti-gst monoclonal antibody  (Rockland Immunochemicals)
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Rockland Immunochemicals dylight 550 conjugated anti-gst monoclonal antibody
The schematic diagram of this study. Yeast proteome microarrays were fabricated from the ~5800 yeast proteins which were individually expressed and purified from yeast. To identify the protein targets, the fabricated yeast proteome microarrays were probed with biotinylated Lfcin B and Histatin-5, individually. Followed by the probing of DyLight 650 labeled streptavidin and <t>DyLight</t> <t>550</t> labeled anti-GST antibody on the yeast proteome microarrays to detect the signal of biotinylated AMPs bound to their specific binding partners and the amount of relative protein on proteome microarray, respectively. The identified hits of Lfcin B and Histatin-5 were systematically analyzed for GO and synthetic lethal pairs by using several online bioinformatics databases (DAVID tools and Synthetic Lethality).
Dylight 550 Conjugated Anti Gst Monoclonal Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ultracel-10k centrifugal filters  (Millipore)
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Millipore ultracel-10k centrifugal filters
The schematic diagram of this study. Yeast proteome microarrays were fabricated from the ~5800 yeast proteins which were individually expressed and purified from yeast. To identify the protein targets, the fabricated yeast proteome microarrays were probed with biotinylated Lfcin B and Histatin-5, individually. Followed by the probing of DyLight 650 labeled streptavidin and <t>DyLight</t> <t>550</t> labeled anti-GST antibody on the yeast proteome microarrays to detect the signal of biotinylated AMPs bound to their specific binding partners and the amount of relative protein on proteome microarray, respectively. The identified hits of Lfcin B and Histatin-5 were systematically analyzed for GO and synthetic lethal pairs by using several online bioinformatics databases (DAVID tools and Synthetic Lethality).
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m per mammalian protein extraction  (Thermo Fisher)
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Thermo Fisher m per mammalian protein extraction
The schematic diagram of this study. Yeast proteome microarrays were fabricated from the ~5800 yeast proteins which were individually expressed and purified from yeast. To identify the protein targets, the fabricated yeast proteome microarrays were probed with biotinylated Lfcin B and Histatin-5, individually. Followed by the probing of DyLight 650 labeled streptavidin and <t>DyLight</t> <t>550</t> labeled anti-GST antibody on the yeast proteome microarrays to detect the signal of biotinylated AMPs bound to their specific binding partners and the amount of relative protein on proteome microarray, respectively. The identified hits of Lfcin B and Histatin-5 were systematically analyzed for GO and synthetic lethal pairs by using several online bioinformatics databases (DAVID tools and Synthetic Lethality).
M Per Mammalian Protein Extraction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human tissue microarray 1  (Millipore)
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Millipore human tissue microarray 1
The schematic diagram of this study. Yeast proteome microarrays were fabricated from the ~5800 yeast proteins which were individually expressed and purified from yeast. To identify the protein targets, the fabricated yeast proteome microarrays were probed with biotinylated Lfcin B and Histatin-5, individually. Followed by the probing of DyLight 650 labeled streptavidin and <t>DyLight</t> <t>550</t> labeled anti-GST antibody on the yeast proteome microarrays to detect the signal of biotinylated AMPs bound to their specific binding partners and the amount of relative protein on proteome microarray, respectively. The identified hits of Lfcin B and Histatin-5 were systematically analyzed for GO and synthetic lethal pairs by using several online bioinformatics databases (DAVID tools and Synthetic Lethality).
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hsp70 interacting protein chip  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc hsp70 interacting protein chip
The schematic diagram of this study. Yeast proteome microarrays were fabricated from the ~5800 yeast proteins which were individually expressed and purified from yeast. To identify the protein targets, the fabricated yeast proteome microarrays were probed with biotinylated Lfcin B and Histatin-5, individually. Followed by the probing of DyLight 650 labeled streptavidin and <t>DyLight</t> <t>550</t> labeled anti-GST antibody on the yeast proteome microarrays to detect the signal of biotinylated AMPs bound to their specific binding partners and the amount of relative protein on proteome microarray, respectively. The identified hits of Lfcin B and Histatin-5 were systematically analyzed for GO and synthetic lethal pairs by using several online bioinformatics databases (DAVID tools and Synthetic Lethality).
Hsp70 Interacting Protein Chip, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The schematic diagram of this study. Yeast proteome microarrays were fabricated from the ~5800 yeast proteins which were individually expressed and purified from yeast. To identify the protein targets, the fabricated yeast proteome microarrays were probed with biotinylated Lfcin B and Histatin-5, individually. Followed by the probing of DyLight 650 labeled streptavidin and DyLight 550 labeled anti-GST antibody on the yeast proteome microarrays to detect the signal of biotinylated AMPs bound to their specific binding partners and the amount of relative protein on proteome microarray, respectively. The identified hits of Lfcin B and Histatin-5 were systematically analyzed for GO and synthetic lethal pairs by using several online bioinformatics databases (DAVID tools and Synthetic Lethality).

Journal: International Journal of Molecular Sciences

Article Title: Systematical Analysis of the Protein Targets of Lactoferricin B and Histatin-5 Using Yeast Proteome Microarrays

doi: 10.3390/ijms20174218

Figure Lengend Snippet: The schematic diagram of this study. Yeast proteome microarrays were fabricated from the ~5800 yeast proteins which were individually expressed and purified from yeast. To identify the protein targets, the fabricated yeast proteome microarrays were probed with biotinylated Lfcin B and Histatin-5, individually. Followed by the probing of DyLight 650 labeled streptavidin and DyLight 550 labeled anti-GST antibody on the yeast proteome microarrays to detect the signal of biotinylated AMPs bound to their specific binding partners and the amount of relative protein on proteome microarray, respectively. The identified hits of Lfcin B and Histatin-5 were systematically analyzed for GO and synthetic lethal pairs by using several online bioinformatics databases (DAVID tools and Synthetic Lethality).

Article Snippet: To monitor the shape, size, and uniformity for each protein spot on a chip, DyLight 550 conjugated anti-GST monoclonal antibody (Rockland Immunochemicals, Gilbertsville, PA, USA) was probed, washed and scanned with LuxScan TM (10K Microarray Scanner; CapitalBio Corporation, Beijing, China).

Techniques: Purification, Labeling, Binding Assay, Microarray

The representative yeast proteome microarray images and the representative hits of Lfcin B and Histatin-5 on yeast proteome microarrays. Images show the microarray and the enlarge hit image of Lfcin B and Histatin-5. Red and green color denotes the signal from DyLight 650 labeled streptavidin and DyLight 550 labeled anti-GST antibody, respectively. Red spots represent the signal of Lfcin B and Histatin-5 on yeast proteome microarrays. ( A ) Triplicate microarray images probed with biotinylated Lfcin B. The position of the representative 5 hits image is shown on the microarray. ( B ) Representative enlarged hits images of Lfcin B. ( C ) Triplicate microarray images probed with biotinylated Histatin-5. ( D ) Representative enlarged hits images of Histatin-5.

Journal: International Journal of Molecular Sciences

Article Title: Systematical Analysis of the Protein Targets of Lactoferricin B and Histatin-5 Using Yeast Proteome Microarrays

doi: 10.3390/ijms20174218

Figure Lengend Snippet: The representative yeast proteome microarray images and the representative hits of Lfcin B and Histatin-5 on yeast proteome microarrays. Images show the microarray and the enlarge hit image of Lfcin B and Histatin-5. Red and green color denotes the signal from DyLight 650 labeled streptavidin and DyLight 550 labeled anti-GST antibody, respectively. Red spots represent the signal of Lfcin B and Histatin-5 on yeast proteome microarrays. ( A ) Triplicate microarray images probed with biotinylated Lfcin B. The position of the representative 5 hits image is shown on the microarray. ( B ) Representative enlarged hits images of Lfcin B. ( C ) Triplicate microarray images probed with biotinylated Histatin-5. ( D ) Representative enlarged hits images of Histatin-5.

Article Snippet: To monitor the shape, size, and uniformity for each protein spot on a chip, DyLight 550 conjugated anti-GST monoclonal antibody (Rockland Immunochemicals, Gilbertsville, PA, USA) was probed, washed and scanned with LuxScan TM (10K Microarray Scanner; CapitalBio Corporation, Beijing, China).

Techniques: Microarray, Labeling